UniProtKB/Swiss-Prot PTM Description

The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa, Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin, N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor, Characterization of O-linked glycan was studied in Bowes melanoma cell line

Glycosylation Sites

Position Structures Description Evidence
ASN-152, ASN-219, ASN-483 Associated Structures: 20 global GlycoSuite
Position Structures Description Evidence
ASN-219 OF P00750, ASN-322 OF P00749 Associated Structures: 16 global GlycoSuite
Position Structures Description Evidence
THR-96, ASN-152, ASN-219, ASN-483 Associated Structures: 15 global GlycoSuite
Site-Specific Information

A number of glycan structures have been assigned to specific glycosylation sites

Position Structures Description Evidence
ASN-322 OF P00749 Associated Structures: 13 Site specific GlycoSuite
ASN-219 AND ASN-483 Associated Structures: 6 Site specific GlycoSuite
THR-96 Associated Structures: 1 Site specific GlycoSuite
ASN-322 OF P00749 Associated Structures: 0 Site specific GlycoSuite

Notes

Accompanying information sourced from GlycoSuiteDB

  1. ASN-152, ASN-219{partial} AND ASN-483 [PFEIFFER ET AL. (1989) EUR. J. BIOCHEM. 186:273-286
  2. THR-96 [HARRIS ET AL. (1991) BIOCHEMISTRY 30:2311-2314
  3. ASN-152, ASN-219 AND ASN-483 [POHL ET AL. (1987) EUR. J. BIOCHEM. 170:69-75

Sequence

MDAMKRGLCCVLLLCGAVFVSPSQEIHARFRRGARSYQVICRDEKTQMIYQQHQSWLRPVLRSNRVEYCWCNSGRAQCHSVPVKSCSEPRCFNGGTCQQALYFSDFVCQCPEGFAGKCCEIDTRATCYEDQGISYRGTWSTAESGAECTNWNSSALAQKPYSGRRPDAIRLGLGNHNYCRNPDRDSKPWCYVFKAGKYSSEFCSTPACSEGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP